Difference between revisions of "Part:BBa K907004"

Revision as of 16:12, 25 September 2012

Dual phase protein generator(GFP default). mRFP and GFP

This part is derivative of BBa_K907002.

BBa_E1010(mRFP, reversed) and BBa_E0040(wtGFP) are attached to both ends of BBa_K907002 by overlapping extension PCR. This part normally generates GFP in E.coli(strain MG1655). When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate mRFP.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 756
Illegal NheI site found at 779
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 838
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 703
Illegal AgeI site found at 10
Illegal AgeI site found at 122
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 723
Illegal BsaI.rc site found at 806
Illegal BsaI.rc site found at 1499

@@ Line 6: / Line 6: @@
 This part is derivative of BBa_K907002.
-BBa_E1010(mRFP, reversed) and BBa_E0040(wtGFP) are attached both end of BBa_K907002 with overlapping extension PCR.
+BBa_E1010(mRFP, reversed) and BBa_E0040(wtGFP) are attached to both ends of BBa_K907002 by overlapping extension PCR.
-This part generates GFP in ''E.coli(strain MG1655)'' normally.
+This part normally generates GFP in ''E.coli(strain MG1655)''.
 When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate mRFP.