Difference between revisions of "Part:BBa K784039"

Latest revision as of 16:08, 25 September 2012

T7*(K1F) RNA polymerase + terminator

This part includes T7*(K1F) RNA Polymerase gene and double terminator (BBa_B0015). This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells.The part was made by cloning the T7*(K1F) RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3.

Note: the biobrick prefix has been altered to deletion of one bp downstream to the XbaI site. This was due to wrong primers.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K784039 short</partinfo>
-This part has the K1F RNA Polymerase gene and terminator (BBa_B0015). This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells.
+This part includes T7*(K1F) RNA Polymerase gene and double terminator (BBa_B0015). This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells.The part was made by cloning the T7*(K1F) RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3.
+Note: the biobrick prefix has been altered to deletion of one bp downstream to the XbaI site. This was due to wrong primers.
 <!-- Add more about the biology of this part here