Difference between revisions of "Part:BBa K917005:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K917005=== | ===Applications of BBa_K917005=== | ||
| + | Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 15:46, 25 September 2012
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how you used this part and how it worked out.
Applications of BBa_K917005
Expression of this biobrick inhibits growth of cells in the presence of nitro drugs such as metronidazole. We have rigorously tested this biobrick's potential as a counter-selectable marker in its pre-biobrick form (in Blue script vector with lac promoter).Although growth of cells containing nitroreductase is inhibited in plates, it is not absolute and therefore unsuitable for couter-selection. Aerobic liquid culture results are more promising with profound growth inhibition of nitroteductase-containing cells while control cells are growing.
User Reviews
UNIQ7b9e443e4c77ce07-partinfo-00000000-QINU UNIQ7b9e443e4c77ce07-partinfo-00000001-QINU