Difference between revisions of "Part:BBa K886001:Experience"

(Applications of BBa_K886001)
(Applications of BBa_K886001)
 
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===Applications of BBa_K886001===
 
===Applications of BBa_K886001===
  
We used this part for the Plug&Play machine, it worked. It was used as part in a receptor plasmid - it expressed the Cre recombinase enzyme, which inserted the PCR-amplified DNA at the lox71 site.
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We used this part for building a Plug&Play Machine version, this version worked (low copy number backbone - pSB4A5).  
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This Plug&Play Machine version had this part under the T7 promoter control - it expressed the Cre recombinase enzyme, which inserted the PCR-amplified DNA at the lox71 site.
  
 
More details http://2012.igem.org/Team:USP-UNESP-Brazil/Plasmid_Plug_n_Play/Introduction
 
More details http://2012.igem.org/Team:USP-UNESP-Brazil/Plasmid_Plug_n_Play/Introduction

Latest revision as of 15:44, 25 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K886001

We used this part for building a Plug&Play Machine version, this version worked (low copy number backbone - pSB4A5).

This Plug&Play Machine version had this part under the T7 promoter control - it expressed the Cre recombinase enzyme, which inserted the PCR-amplified DNA at the lox71 site.

More details http://2012.igem.org/Team:USP-UNESP-Brazil/Plasmid_Plug_n_Play/Introduction

User Reviews

UNIQ630a5e26cb80f09f-partinfo-00000000-QINU UNIQ630a5e26cb80f09f-partinfo-00000001-QINU