Difference between revisions of "Part:BBa K883701:Design"

 
 
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__NOTOC__
 
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<partinfo>BBa_K883701 short</partinfo>
 
<partinfo>BBa_K883701 short</partinfo>
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===Design Notes===
 
===Design Notes===
...
 
  
 +
To be able to use the device, the counterpart of the E-coil needs to be fused to the protein that needs to be detected.
  
  
===Source===
 
  
...
+
{| class="wikitable" style="text-align: center"
 +
|- style="font-style: italic"
 +
|name
 +
|sequence 
 +
|translated 
 +
|-
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|K-coil (counterpart)
 +
|aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag   
 +
|KIAALKEKIAALKEKIAALKE     
 +
|-
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|E-coil (present in the brick)   
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|gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa     
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|EIAALEKEIAALEKEIAALEK     
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|}
 +
 
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===Source===
 +
The biobrick [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] was used as a template for the mutations
  
 
===References===
 
===References===

Latest revision as of 15:23, 25 September 2012

GFP with a His-tag fused to a coiled coil


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 710


Design Notes

To be able to use the device, the counterpart of the E-coil needs to be fused to the protein that needs to be detected.


name sequence translated
K-coil (counterpart) aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag KIAALKEKIAALKEKIAALKE
E-coil (present in the brick) gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa EIAALEKEIAALEKEIAALEK

Source

The biobrick BBa_I13522 was used as a template for the mutations

References