Difference between revisions of "Part:BBa K750012"

Revision as of 15:12, 25 September 2012

TIME DELAY0.01:LuxI(RBS0.01)->LuxR->LuxPR->GFP

This part contains BBa_K750004 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI will synthesize a small, diffusible signaling molecule acyl-homoserinelactone(AHL). The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of our reporter GFP under the control of a promoter lux pR. Expression of LuxI will be affected when strength of RBS before LuxI gene is changed. When expression of LuxI is weak, speed of AHL production will go down, that means it costs longer time for AHL-LuxR to arrive the activation thrshold of the promoter LuxPR. In the time delay part of our project E.Lumoli, we designed 4 circuit with RBSes of different strength in the LuxI producer to construct a time delay device. Other time delay parts: TD1.0(BBa_K750009), TD0.6(BBa_K750010), TD0.3(BBa_K750011).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
Illegal NheI site found at 1068
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 792
Illegal BamHI site found at 65
Illegal BamHI site found at 1008
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2023
Illegal BsaI.rc site found at 2750

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K750012 short</partinfo>
-This part contains part8 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.
+This part contains BBa_K750004 and BBa_K750007. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI will synthesize a small, diffusible signaling molecule acyl-homoserinelactone(AHL). The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of our reporter GFP under the control of a promoter lux pR. Expression of LuxI will be affected when strength of RBS before LuxI gene is changed. When expression of LuxI is weak, speed of AHL production will go down, that means it costs longer time for AHL-LuxR to arrive the activation thrshold of the promoter LuxPR. In the time delay part of our project E.Lumoli, we designed 4 circuit with RBSes of different strength in the LuxI producer to construct a time delay device. Other time delay parts: TD1.0(BBa_K750009), TD0.6(BBa_K750010), TD0.3(BBa_K750011).
 <!-- Add more about the biology of this part here