Difference between revisions of "Part:BBa K738000"
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<partinfo>BBa_K738000 short</partinfo> | <partinfo>BBa_K738000 short</partinfo> | ||
| − | Scaffold D0 was constructed from a single RNA module d0, which folded into a duplex with PP7 and MS2 aptamer domains that bind PP7 and MS2 fusion proteins | + | Scaffold D0 was constructed from a single RNA module d0, which folded into a duplex with PP7 and MS2 aptamer domains that bind PP7 and MS2 fusion proteins. |
| + | [[Image:Fluorescence protein complementation.jpg]] | ||
| + | GFP split into two halves (Fa and Fb), each of which is fused to MS2 or PP7. Fa and Fb then bind to their respective aptamers. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 14:40, 25 September 2012
RNA Scaffold generator
Scaffold D0 was constructed from a single RNA module d0, which folded into a duplex with PP7 and MS2 aptamer domains that bind PP7 and MS2 fusion proteins.
GFP split into two halves (Fa and Fb), each of which is fused to MS2 or PP7. Fa and Fb then bind to their respective aptamers.
Usage and Biology
In cells, multienzymatic pathways are often physically and spatially organized onto scaffolds or clusters or into microcompartments. Spatial organization helps substrates flow between interacting proteins, limits cross-talk between signaling pathways, and increases yields of sequential metabolic reactions. But multi-enzyme pathways in vivo widely used in chemical and medical industry are not naturally. So there is lacking in relative scaffolds used as spatial organization. BBa_K738000 is a discrete engineered RNA module for controling the spatial organization of bound proteins, which is able to make the production of the multi-enzyme pathways more efficient.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]