Difference between revisions of "Part:BBa K883701:Design"

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===Design Notes===
 
===Design Notes===
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To be able to use the device, the counterpart of the E-coil needs to be fused to the protein that needs to be detected.
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{| class="wikitable" style="text-align: center"
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|- style="font-style: italic"
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|name
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|sequence 
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|translated 
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|-
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|E-coil (present in the brick)
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|aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag   
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|KIAALKEKIAALKEKIAALKE     
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|-
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|K-coil (counterpart)     
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|gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa     
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|EIAALEKEIAALEKEIAALEK     
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|}
  
 
===Source===
 
===Source===

Revision as of 13:40, 25 September 2012

GFP with a His-tag fused to a coiled coil


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 710


Design Notes

To be able to use the device, the counterpart of the E-coil needs to be fused to the protein that needs to be detected.


name sequence translated
E-coil (present in the brick) aagatagcggcgttgaaggagaaaatcgcagcactaaaagaaaagatagcggcgttgaaggag KIAALKEKIAALKEKIAALKE
K-coil (counterpart) gaaattgcggcgctggaaaaagaaattgcggcgctggaaaaagaaattgcggcgctggaaaaa EIAALEKEIAALEKEIAALEK

Source

The biobrick BBa_I13522 was used as a template for the mutations

References