Difference between revisions of "Part:BBa K782004"

Revision as of 12:58, 25 September 2012

TALA:NLS DNA binding domain

TALA label represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011).

Introduction

TAL effectors (TALEs) are bacterial plant pathogen transcription factors, that bind to DNA by specifically recognizing one base pair with a single tandem repeat in their DNA-binding domain. A tandem TALE repeat contains 33 to 35 amino acids, where the 12th and 13th amino acid, called a “repeat variable diresidue” (RVD), are responsible for specific interactions with the corresponding base pair (Scholze and Boch, 2011).

Figure 1: Schematic representation of the construct

Characterization

TAL effector was obtained via AddGene from Joung's lab and cloned into a biobrick vector. This construct was later used for designing TAL-based activator and repressor by adding VP16 [1] and KRAB [2] domain.

Single binding sequence for TALA: TTTACTGCTGCTCCCGCT

References

Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698 Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.

Scholze, H., and Boch, J. (2011) TAL effectors are remote controls for gene activation. Curr. Opin. Microbiol. 14, 47-53.

Sequence and Features