Difference between revisions of "Part:BBa K784038"
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<partinfo>BBa_K784038 short</partinfo> | <partinfo>BBa_K784038 short</partinfo> | ||
| − | This part has the K1F RNA Polymerase gene. This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells. | + | This part has the K1F RNA Polymerase gene. This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells. This part was recieved from Chris Voight lab (see sources). In order to achieve reduced levels of toxicity Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and GTG start codon. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 12:14, 25 September 2012
T7*(K1F) RNA polymerase
This part has the K1F RNA Polymerase gene. This is orthogonal polymerases based on T7 wt RNAP that is less toxic to host cells. This part was recieved from Chris Voight lab (see sources). In order to achieve reduced levels of toxicity Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and GTG start codon. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]