Difference between revisions of "Part:BBa K750111"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K750111 short</partinfo> | <partinfo>BBa_K750111 short</partinfo> | ||
− | + | == Description == | |
+ | This part includes TetR composition, promoter Pter, RBS of 1.0 strength, a unstable GFP reporter and the double terminator. It is designed to work when (anhydro)tetracyclin is added. In the digital display part of our project E.Lumoli, we need different induction to activate the circuit and make GFP be produced or be degraded. The inspiration of this part comes from BBa_K145280(designed by iGEM08_KULeuven). We planed to use this biobrick in our project at the very start, but when we test this device by using 140 ng/ul atc(protocol from team KULeuven), the device could not work. So we changed the RBS from 0.3 strength to 1.0 strength. This time, the device worked. | ||
+ | |||
+ | == performance == | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 11:49, 25 September 2012
PtetGLT:GFP(lva) expression system controlled by (anhydro)tetracyclin
Description
This part includes TetR composition, promoter Pter, RBS of 1.0 strength, a unstable GFP reporter and the double terminator. It is designed to work when (anhydro)tetracyclin is added. In the digital display part of our project E.Lumoli, we need different induction to activate the circuit and make GFP be produced or be degraded. The inspiration of this part comes from BBa_K145280(designed by iGEM08_KULeuven). We planed to use this biobrick in our project at the very start, but when we test this device by using 140 ng/ul atc(protocol from team KULeuven), the device could not work. So we changed the RBS from 0.3 strength to 1.0 strength. This time, the device worked.
performance
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1615