Difference between revisions of "Part:BBa K929004:Design"
(→Design Notes) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
[[Image:UP_12_designinfo_BBa_K929004.png|center|thumb|600px]] | [[Image:UP_12_designinfo_BBa_K929004.png|center|thumb|600px]] | ||
| − | The modified AID (BBa_929001) is in RFC 10 and has an aditional AgeI restriction site. Therefore it can be fused with RFC 25 parts at the C-terminus. We cut modified AID (BBa_929001)with AgeI and PstI and eGFP (BBa_K404316) with NgoMIV and PstI and ligatet the fragments. | + | The modified AID (BBa_929001) is in RFC 10 and has an aditional AgeI restriction site. Therefore it can be fused with RFC 25 parts at the C-terminus. We cut modified AID (BBa_929001)with AgeI and PstI and eGFP (BBa_K404316) with NgoMIV and PstI and ligatet the fragments. The resulting Biobrick ([https://parts.igem.org/part:BBa_K929004 BBa_K929004]) is in RFC 10 and has also an aditional AgeI restriction site in front of the |
| + | stop codon taa. Therefore further fusion(C-terminal of ([https://parts.igem.org/part:BBa_K929004 BBa_K929004] with RFC 25 parts is possible. | ||
===Source=== | ===Source=== | ||
Revision as of 09:59, 25 September 2012
modified AID+eGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1296
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 108
Illegal BsaI.rc site found at 1225
Illegal SapI site found at 209
Design Notes
The modified AID (BBa_929001) is in RFC 10 and has an aditional AgeI restriction site. Therefore it can be fused with RFC 25 parts at the C-terminus. We cut modified AID (BBa_929001)with AgeI and PstI and eGFP (BBa_K404316) with NgoMIV and PstI and ligatet the fragments. The resulting Biobrick (BBa_K929004) is in RFC 10 and has also an aditional AgeI restriction site in front of the stop codon taa. Therefore further fusion(C-terminal of (BBa_K929004 with RFC 25 parts is possible.
Source
This is a composite part from the existing parts: modified_AID (BBa_929001) and eGFP (BBa_K404316). We used RFC 25 enzymes to create this part as a fusion of modified AID and eGFP (at the C-terminus of mod_AID).