Difference between revisions of "Part:BBa K864200:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K864200 short</partinfo>
 
<partinfo>BBa_K864200 short</partinfo>
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===Source===
 
===Source===
  
Amplified from MG1655 (Accession NC_000913) chromosome using primers listed below  
+
Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are <u>underlined</u>)
  
 
marR_BB_F
 
marR_BB_F
TTCGAATTCGCGGCCGCTTCTAGATGAAAAGTACCAGCGATCT
+
TTCGAATTCGCGGCCGCTTCTAGA<u>TGAAAAGTACCAGCGATCT</u>
  
 
marR_BB_R
 
marR_BB_R
GGCCGCTACTAGTATTATTACGGCAGGACTTTCTT
+
GGCCGCTACTAGTATTA<u>TTACGGCAGGACTTTCTT</u>
  
 
===References===
 
===References===

Latest revision as of 09:58, 25 September 2012

MarR native E. coli repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

Amplified from MG1655 (accession NC_000913) chromosome using primers listed below (annealing sequences are underlined)

marR_BB_F TTCGAATTCGCGGCCGCTTCTAGATGAAAAGTACCAGCGATCT

marR_BB_R GGCCGCTACTAGTATTATTACGGCAGGACTTTCTT

References