Difference between revisions of "Part:BBa K819008:Design"

 
(→‎Design Notes)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K819008 short</partinfo>
 
<partinfo>BBa_K819008 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
luxABCDE gene ligated to the rear of T7 promoter. The original Ara and P-bad were replaced with T7 promoter, which enables its usage in our project.
+
Lux CDABEG genes and related RBS from [https://parts.igem.org/Part:BBa_K325909:Design BBa_K325909] are placed under T7 promoter [https://parts.igem.org/Part:BBa_I712074 BBa_K712074].
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 08:50, 25 September 2012

Lux Operon under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2012
    Illegal XhoI site found at 2842
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4401
    Illegal BsaI.rc site found at 1410
    Illegal SapI.rc site found at 4726


Design Notes

Lux CDABEG genes and related RBS from BBa_K325909 are placed under T7 promoter BBa_K712074.

Source

T7 promoter and LuxCDABE with their RBS are from Partsregistry.

References