Difference between revisions of "Part:BBa K808002"

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tctA_503: large subunit A1 of the tripartite tricarboxylate transporter family

The large subunit A1 of the tripartite tricarboxylate transporter family (tctA_503, 52,98 kDa) was isolated from Comamonas testosteroni KF-1. The tripartite tricarboxylate transporter system consists of three different proteins: a periplasmatic solute binding receptor, a membrane protein with 12 putative transmembrane alpha-helical spanners (in this case tctA_503), and a small poorly conserved membrane proteine with four putative transmembrane alpha-helical spanner^[1]..The strain was purchased from Leibniz Institute DMSZ-German Collection of Microorganism and Cell Cultures (DMSZ no. 14576). The original sequence contains a Pst1 recognition site. To eliminate this recognition site a directed-site mutagenic PCR was performed. (For more datails: link zu dem PCR protokoll und dem Labjournal, wo isolations PCR beschrieben wird) To characterized the structure of the tctA_503 bioinformatic tools like Protein Homology/anologY Recognition Engine V 2.0 (PHYRE2), I-TASSER servers, and TMHMM was used. The TMHMM predicted a transmembrane protein with 11 alpha-helical spanners (Fig. 1). The N-teminus is with a probability of 98 % in cytoplasmatic. The NCBI Protein BLAST results shows that the tctA_503 subunit A1 belongs to the tctA superfamily. PHYRE2 and I-Tasser server homology modelling did not give a significant result for the structure of the tctA_503 subunit A1.

Figure 1. TMHMM prediction of the tctA_503 subunit A1. It shows 11 alpha-helical spanners.

Sequence and Features

References

[1] Sasoh, M., E. Masai, et al. (2006). "Characterization of the terephthalate degradation genes of Comamonas sp. strain E6." Appl Environ Microbiol 72(3): 1825-1832.

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