Difference between revisions of "Part:BBa K819005"
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a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass photosensor protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and M135I. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes with their promoter containing mutated 408 form of SOS box. | a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass photosensor protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and M135I. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes with their promoter containing mutated 408 form of SOS box. | ||
| + | ''Luminesensor'' is designed by following the general principle of optogenetics fusion protein design--attaching a physiologically functional domain to a photoreceptor domain. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:44, 25 September 2012
Constitutive LuxBrick Generator
a fusion protein consisting of E.coli endogenous SOS system repressor LexA and fungus N.crass photosensor protein VVD with LexA carrying mutation at position 40-42 and VVD carrying mutation N56K, C71V and M135I. This part serves as an ultra sensitive photoreceptor(which can sense light as weak as moonlight) and will induce a light-dependent repression of genes with their promoter containing mutated 408 form of SOS box.
Luminesensor is designed by following the general principle of optogenetics fusion protein design--attaching a physiologically functional domain to a photoreceptor domain. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3014
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2012
Illegal XhoI site found at 2842 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 4401
Illegal BsaI.rc site found at 1410
Illegal SapI.rc site found at 4726