Difference between revisions of "Part:BBa K792003"

Revision as of 07:20, 25 September 2012

Import enhancer - HIV TAT penetratin (trojan peptide)

Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell.

This part is the penetratin from the HIV TAT protein, and has to be used just before the coding sequence of the peptide that you want to be import enhanced.

This part includes a HindIII restriction site as an extra.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K792003 short</partinfo>
-Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process [Derossi 1998]. They can be used to increase the efficiency with which a protein enters a cell.
-This part is the penetratin from the HIV TAT protein, and has to be used just before the coding sequence of the peptide that you want to be ''import enhanced''.
+Trojan peptides are short sequences that penetrate through the plasma membrane inside the cell without the need of any receptor or endocitosis process ''[Derossi 1998]''. They '''can be used to increase the efficiency with which a protein enters a cell'''.
+This part is the '''penetratin from the HIV TAT''' protein, and has to be used just before the coding sequence of the peptide that you want to be ''import enhanced''.
+This part includes a '''HindIII''' restriction site as an extra.
 <!-- Add more about the biology of this part here