Difference between revisions of "Part:BBa K864121"
(→‎Usage and Biology)
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===Usage and Biology===
 
===Usage and Biology===
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Useable as default insert of low and medium copy backbones, especially in lacI positive systems.  
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Usable as default insert of low and medium copy backbones, especially in lacI positive systems. This is the default insert of the pSB4x15Iq and pSB8x15 series of backbones.  
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 06:43, 25 September 2012

pMB1 ori - constitutive RFP

This is a minimal high-copy replication origin coupled to a constitutive RFP casette. Any vector containing K864121 will be propagated at high copy and express a strong red color after 12-18 hours of incubation.


Usage and Biology

Usable as default insert of low and medium copy backbones, especially in lacI positive systems. This is the default insert of the pSB4x15Iq and pSB8x15 series of backbones.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1322
    Illegal AgeI site found at 1434
  • 1000
    COMPATIBLE WITH RFC[1000]