Difference between revisions of "Part:BBa K864502:Design"
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<partinfo>BBa_K864502 short</partinfo> | <partinfo>BBa_K864502 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | The CP44 promoter was chosen to be similar to the LacI promoter. | + | The CP44 promoter was chosen to be similar in strength to the LacI promoter, and to be used as a replacement for that one in the standard RFP cassette. |
| − | + | ||
| + | This part has not been assembled as a stand-alone BioBrick. It is a logical part of the pMB1ori-redq <partinfo>BBa_K864121</partinfo>, to be used as a default insert in low and middle copy backbones. | ||
===Source=== | ===Source=== | ||
| − | A BBa_J04450 RFP reporter, but modified by PCR mutagenesis. | + | A <partinfo>BBa_J04450</partinfo> RFP reporter, but modified by PCR mutagenesis. |
===References=== | ===References=== | ||
Latest revision as of 06:41, 25 September 2012
RFP Coding Device, constitutive
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 640
Illegal AgeI site found at 752 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The CP44 promoter was chosen to be similar in strength to the LacI promoter, and to be used as a replacement for that one in the standard RFP cassette.
This part has not been assembled as a stand-alone BioBrick. It is a logical part of the pMB1ori-redq BBa_K864121, to be used as a default insert in low and middle copy backbones.
Source
A BBa_J04450 RFP reporter, but modified by PCR mutagenesis.