Difference between revisions of "Part:BBa K864121"

 
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<partinfo>BBa_K864121 short</partinfo>
 
<partinfo>BBa_K864121 short</partinfo>
  
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This is a minimal high-copy replication origin coupled to a constitutive RFP casette. Any vector containing K864101 will be propagated at high copy and express a strong red color after 12-18 hours of incubation.
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This is a minimal high-copy replication origin coupled to a constitutive RFP casette. Any vector containing K864121 will be propagated at high copy and express a strong red color after 12-18 hours of incubation.
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===Usage and Biology===
 
===Usage and Biology===
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Useable as default insert of low and medium copy backbones, especially in lacI positive systems.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K864121 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K864121 SequenceAndFeatures</partinfo>

Revision as of 06:40, 25 September 2012

pMB1 ori - constitutive RFP

This is a minimal high-copy replication origin coupled to a constitutive RFP casette. Any vector containing K864121 will be propagated at high copy and express a strong red color after 12-18 hours of incubation.


Usage and Biology

Useable as default insert of low and medium copy backbones, especially in lacI positive systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1322
    Illegal AgeI site found at 1434
  • 1000
    COMPATIBLE WITH RFC[1000]