Difference between revisions of "Part:BBa K821012"

Latest revision as of 05:29, 25 September 2012

PBad(promoter)+orthogonal 16S RNA

Without ara, PBad doesn't work, and no o-16S will be produced. Therefore, the orthogonal pathway is shut down. The green byproduct is synthesized

After adding ara, pBad is induced, producing o-16S. The orthogonal pathway works according to plan, and produce violeicin. We can see the green color disappering

Control group of natural violeicin expression

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2207
Illegal BamHI site found at 1260
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1095
Illegal AgeI site found at 2221
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1077

@@ Line 4: / Line 4: @@
 PBad(promoter)+orthogonal 16S RNA
-[[Image:Tianjin_BioBrick10121.PNG|300px]][[Image:Tianjin_BioBrick10122.PNG|300px]][[Image:Tianjin_BioBrick10123.PNG|300px]]
+[[Image:Tianjin_BioBrick10121.PNG|thumb|500px|center|Without ara, PBad doesn't work, and no o-16S will be produced. Therefore, the orthogonal pathway is shut down. The green byproduct is synthesized]]
+[[Image:Tianjin_BioBrick10122.PNG|thumb|500px|center|After adding ara, pBad is induced, producing o-16S. The orthogonal pathway works according to plan, and produce violeicin. We can see the green color disappering]]
+[[Image:Tianjin_BioBrick10123.PNG|thumb|500px|center|Control group of natural violeicin expression]]
 ===Usage and Biology===