Difference between revisions of "Part:BBa K886001:Design"

 
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<partinfo>BBa_K886001 short</partinfo>
 
<partinfo>BBa_K886001 short</partinfo>
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===Design Notes===
 
===Design Notes===
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We used the lox71 submitted by our group, which correct the part BBa_I718017 and BBa_K537020 when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/
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We used the lox71 submitted by our group BBa_K886000, which corrected the parts BBa_I718017 and BBa_K537020. Those parts are wrong when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/
  
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===Source===
  
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We synthesized the lox71 site, then we assembled it upstream the Cre recombinase gene(BBa_J61047).
  
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===Source===
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===References===
  
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We synthesized the lox71 site and then we assembled it with the Cre recombinase part BBa_J61047 
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/
  
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===References===
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http://2012.igem.org/Team:USP-UNESP-Brazil/Plasmid_Plug_n_Play/Introduction

Latest revision as of 22:41, 24 September 2012

Recombination Device composed by lox71-Cre recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the lox71 submitted by our group BBa_K886000, which corrected the parts BBa_I718017 and BBa_K537020. Those parts are wrong when compared with the published sequence at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/

Source

We synthesized the lox71 site, then we assembled it upstream the Cre recombinase gene(BBa_J61047).

References

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC137435/

http://2012.igem.org/Team:USP-UNESP-Brazil/Plasmid_Plug_n_Play/Introduction