Difference between revisions of "Part:BBa K777000"
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K777000 short</partinfo>
 
<partinfo>BBa_K777000 short</partinfo>
−
 
+
[[Image:TAR_structure2.png|thumb|right|300px|'''Fig. 1:''' Tar chemoreceptor 3D structure.]]
 
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
 
The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
  
−
<!-- Add more about the biology of this part here
+
Figure 1 represents the non quikchanged Tar chemoreceptor binding aspartate within its binding site and was constructed using the software PYMOL (PDB ID:1WAT).
 +
<br><br><br>
 +
<br><br><br>
 +
<br><br><br>
 +
 
 +
 
 
===Usage and Biology===
 
===Usage and Biology===
 
This part can be used for chemotaxis assays.
 
This part can be used for chemotaxis assays.
−
 
+
* Here is the complete sequence of this part as an [https://static.igem.org/mediawiki/2012/f/fc/Tar_receptor_under_the_control_of_constitutive_promoter.zip ApE file].
 +
<br>
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
Line 13: Line 19:
 
<br>
 
<br>
 
[[Image:K777000_in_pSB1C3.png|frame|left|'''Fig. 2:''' Plasmid map of K777000 in pSB1C3]]
 
[[Image:K777000_in_pSB1C3.png|frame|left|'''Fig. 2:''' Plasmid map of K777000 in pSB1C3]]
−
 
−
===3D structure of Tar chemoreceptor===
 
−
[[Image:TAR_structure2.png|thumb|left|300px|'''Fig. 3:''' Tar chemoreceptor 3D structure.]]
 
−
Figure 1 represents the non quikchanged Tar chemoreceptor binding aspartate within its binding site and was constructed using the software PYMOL (PDB ID:1WAT).
 
  
  

Latest revision as of 21:07, 24 September 2012

Tar receptor

Fig. 1: Tar chemoreceptor 3D structure.

The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer.

Figure 1 represents the non quikchanged Tar chemoreceptor binding aspartate within its binding site and was constructed using the software PYMOL (PDB ID:1WAT).









Usage and Biology

This part can be used for chemotaxis assays.

  • Here is the complete sequence of this part as an ApE file.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Fig. 2: Plasmid map of K777000 in pSB1C3