Difference between revisions of "Part:BBa K777000"
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<partinfo>BBa_K777000 short</partinfo> | <partinfo>BBa_K777000 short</partinfo> | ||
+ | [[Image:TAR_structure2.png|thumb|right|300px|'''Fig. 1:''' Tar chemoreceptor 3D structure.]] | ||
+ | The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer. | ||
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+ | Figure 1 represents the non quikchanged Tar chemoreceptor binding aspartate within its binding site and was constructed using the software PYMOL (PDB ID:1WAT). | ||
+ | <br><br><br> | ||
+ | <br><br><br> | ||
+ | <br><br><br> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part can be used for chemotaxis assays. | This part can be used for chemotaxis assays. | ||
− | + | * Here is the complete sequence of this part as an [https://static.igem.org/mediawiki/2012/f/fc/Tar_receptor_under_the_control_of_constitutive_promoter.zip ApE file]. | |
+ | <br> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K777000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K777000 SequenceAndFeatures</partinfo> | ||
<br> | <br> | ||
− | + | [[Image:K777000_in_pSB1C3.png|frame|left|'''Fig. 2:''' Plasmid map of K777000 in pSB1C3]] | |
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Latest revision as of 21:07, 24 September 2012
Tar receptor
The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer.
Figure 1 represents the non quikchanged Tar chemoreceptor binding aspartate within its binding site and was constructed using the software PYMOL (PDB ID:1WAT).
Usage and Biology
This part can be used for chemotaxis assays.
- Here is the complete sequence of this part as an ApE file.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1282
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 111