Difference between revisions of "Part:BBa K777000"
 
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<partinfo>BBa_K777000 short</partinfo>
 
<partinfo>BBa_K777000 short</partinfo>
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[[Image:TAR_structure2.png|thumb|right|300px|'''Fig. 1:''' Tar chemoreceptor 3D structure.]]
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The transmembrane chemoreceptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate and exists as a functional homodimer.
  
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The transmembrane receptor Tar of <i>E. coli</i> mediates chemotaxis towards aspartate.
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Figure 1 represents the non quikchanged Tar chemoreceptor binding aspartate within its binding site and was constructed using the software PYMOL (PDB ID:1WAT).
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<br><br><br>
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<!-- Add more about the biology of this part here
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<br><br><br>
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===Usage and Biology===
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<br><br><br>
  
  
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===Usage and Biology===
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This part can be used for chemotaxis assays.
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* Here is the complete sequence of this part as an [https://static.igem.org/mediawiki/2012/f/fc/Tar_receptor_under_the_control_of_constitutive_promoter.zip ApE file].
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K777000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K777000 SequenceAndFeatures</partinfo>
 
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[[Image:K777000_in_pSB1C3.png|frame|left|'''Fig. 2:''' Plasmid map of K777000 in pSB1C3]]
  
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===3D structure of Tar receptor===
 
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[[Image:TAR_structure2.png|thumb|left|300px|'''Fig. 1:'''Tar receptor 3D structure]]
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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Latest revision as of 21:07, 24 September 2012

Tar receptor

Fig. 1: Tar chemoreceptor 3D structure.

The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer.

Figure 1 represents the non quikchanged Tar chemoreceptor binding aspartate within its binding site and was constructed using the software PYMOL (PDB ID:1WAT).









Usage and Biology

This part can be used for chemotaxis assays.

  • Here is the complete sequence of this part as an ApE file.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Fig. 2: Plasmid map of K777000 in pSB1C3