Difference between revisions of "Part:BBa K743014"

Latest revision as of 20:09, 24 September 2012

after dusk -> LuxAB luciferase (Photorabdus luminiscens)

This part is designed for the expression of the LuxA and LuxB genes from Photorabdus luminiscens in a circadian manner with peak 2 hours after subjective dusk (or hour 14 of circadian time).

When transformed into Synechocystis PCC6803, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time.

This part allows the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 654
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1173

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K743014 short</partinfo>
-This part is designed for the expression of the LuxA and LuxB genes from Photorabdus luminiscens in a circadian manner, peaking a 2 hours after subjective dusk (or hour 14 of circadian time if you prefer).
+This part is designed for the expression of the LuxA and LuxB genes from <i>Photorabdus luminiscens</i> in a circadian manner with peak 2 hours after subjective dusk (or hour 14 of circadian time).
-When transfromed into Syenchocystis PCC6803, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time.
-This permits for the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates)
+When transformed into <i>Synechocystis PCC6803</i>, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time.
+This part allows the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates).
+[[Image:placa lux p.l.jpg|600px]]
-[[Image:placa lux p.l.jpg]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===