Difference between revisions of "Part:BBa K743013"

 
 
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__NOTOC__
 
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<partinfo>BBa_K743013 short</partinfo>
 
<partinfo>BBa_K743013 short</partinfo>
  
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'''OmpA - HIV autocleavage secretion system'''
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Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A  fused to a extracellular linker ([https://parts.igem.org/Part:BBa_K103006 ompA brick]) followed by a [https://parts.igem.org/Part:BBa_I712015 cleavage site] recognizable by [https://parts.igem.org/Part:BBa_I712667 HIV protease] which is linked to that protease coding sequence.
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We expect the protease recognizes its own cleavage site and releases itself to the medium.
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This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP.
  
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You can check the results HERE LINK!
 
   
 
   
  

Latest revision as of 20:03, 24 September 2012

Arabinose --> HIV protease secretion

OmpA - HIV autocleavage secretion system

Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker (ompA brick) followed by a cleavage site recognizable by HIV protease which is linked to that protease coding sequence.

We expect the protease recognizes its own cleavage site and releases itself to the medium.

This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP.

You can check the results HERE LINK!


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961