Difference between revisions of "Part:BBa K743012"

 
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'''Arabinose -> extracellular GFP'''
 
'''Arabinose -> extracellular GFP'''
  
Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A  fused to a extracellular linker ([https://parts.igem.org/Part:BBa_K103006 ompA brick]) followed by a cleavage site recognizable by [https://parts.igem.org/Part:BBa_I712667 HIV protease] wich is linked to GFP coding sequence.
+
Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A  fused to a extracellular linker ([https://parts.igem.org/Part:BBa_K103006 ompA brick]) followed by a cleavage site recognizable by [https://parts.igem.org/Part:BBa_I712667 HIV protease] which is linked to GFP coding sequence.
  
If HIV protease is present in the medium, it will cut the cleavage site and GFP will be released from the outer membrane
+
If HIV protease is present in the medium, it will cut the cleavage site and GFP will be released from the outer membrane.
  
It was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP.
+
This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP.
 
You can check the results HERE LINK!
 
You can check the results HERE LINK!
  

Latest revision as of 20:01, 24 September 2012

OmpA + HIV protease cleavage site - GFP secretion system

Arabinose -> extracellular GFP

Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker (ompA brick) followed by a cleavage site recognizable by HIV protease which is linked to GFP coding sequence.

If HIV protease is present in the medium, it will cut the cleavage site and GFP will be released from the outer membrane.

This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP. You can check the results HERE LINK!


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1743