Difference between revisions of "Part:BBa K743012"
Juan Alamos (Talk | contribs) |
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'''Arabinose -> extracellular GFP''' | '''Arabinose -> extracellular GFP''' | ||
− | Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker ([https://parts.igem.org/Part:BBa_K103006 ompA brick]) followed by a cleavage site recognizable by [https://parts.igem.org/Part:BBa_I712667 HIV protease] | + | Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker ([https://parts.igem.org/Part:BBa_K103006 ompA brick]) followed by a cleavage site recognizable by [https://parts.igem.org/Part:BBa_I712667 HIV protease] which is linked to GFP coding sequence. |
− | If HIV protease is present in the medium, it will cut the cleavage site and GFP will be released from the outer membrane | + | If HIV protease is present in the medium, it will cut the cleavage site and GFP will be released from the outer membrane. |
− | + | This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP. | |
You can check the results HERE LINK! | You can check the results HERE LINK! | ||
Latest revision as of 20:01, 24 September 2012
OmpA + HIV protease cleavage site - GFP secretion system
Arabinose -> extracellular GFP
Upon addition of arabinose to the medium, the inducible Pbad promoter drives the expression of outer membrane protein A fused to a extracellular linker (ompA brick) followed by a cleavage site recognizable by HIV protease which is linked to GFP coding sequence.
If HIV protease is present in the medium, it will cut the cleavage site and GFP will be released from the outer membrane.
This part was used by [http://2012.igem.org/Team:UC_Chile 2012 UC Chile team] to secrete GFP. You can check the results HERE LINK!
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 1743