Difference between revisions of "Part:BBa K743006"

Latest revision as of 19:32, 24 September 2012

psb1C3_IntK recombination plasmid for Synechocystis PCC6803

This plasmid is intended to be used as a starting backbone for further addition of DNA parts by Gibson assembly between RS1 and KanR. As Synechocystis PCC6803 undergoes double homologous recombination naturally , any sequence between RS1 and RS2 will be introduced into its chromosome along with a KanR gene for selection in cyanobacteria or in E. coli.

The plasmid backbone also has a Chloramphenicol resistance casette for selection in E.coli only. The use of BBa_K743000 and BBa_K743001 as recombination sites has no deleterious phenotypic effects on the cells.

This part was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis PCC6803.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1774
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1774
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1774
Illegal XhoI site found at 2271
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1774
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1774
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 511

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K743006 short</partinfo>
-This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome, this allows for selection in this cyanobacteria. It has a Chloramphenicol resistance casette for selection in E.coli
+This plasmid is intended to be used as a starting backbone for further addition of DNA parts by Gibson assembly between RS1 and KanR. As <i>Synechocystis PCC6803</i> undergoes double homologous recombination naturally , any sequence between RS1 and RS2 will be introduced into its chromosome along with a KanR gene for selection in cyanobacteria or in <i>E. coli</i>.
-The use of BBa_K743000 and BBa_K743001 as recombination sites has no phenotypic effects on the cells.
-The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly.
+The plasmid backbone also has a Chloramphenicol resistance casette for selection in <i>E.coli</i> only.
-It was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform Synechocystis
+The use of [https://parts.igem.org/Part:BBa_K743000 BBa_K743000] and [https://parts.igem.org/Part:BBa_K743001 BBa_K743001] as recombination sites has no deleterious phenotypic effects on the cells.
+This part was used by [http://2012.igem.org/Team:UC_Chile UC_Chile 2012 team] to succesfully transform <i>Synechocystis PCC6803</i>.
 <!-- Add more about the biology of this part here