Difference between revisions of "Part:BBa K743005"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K743005 short</partinfo>
 
<partinfo>BBa_K743005 short</partinfo>
  
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This plasmid allows the integration of a Kanamycin resistance casette followed by a double terminator in to Synechocystis chromosome.
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This composite part allows the expression of RFP under <i>Synechocystis PCC6803 </i> psbA2 operon promoter in the [https://parts.igem.org/Part:BBa_K743006 psb1C3_IntK integration plasmid].
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The plasmid is intended to be used as a starting backbone for further adition of dna parts by Gibson assembly.
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It was used by UC_Chile 2012 team to succesfully transform Synechocystis [http://2012.igem.org/Team:UC_Chile]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:28, 24 September 2012

psb1C3_IntK plasmid with RFP under psbA2 promoter

This composite part allows the expression of RFP under Synechocystis PCC6803 psbA2 operon promoter in the psb1C3_IntK integration plasmid.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2684
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2684
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2684
    Illegal XhoI site found at 3181
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2684
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2684
    Illegal AgeI site found at 1178
    Illegal AgeI site found at 1290
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 511