Difference between revisions of "Part:BBa K802004"
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<partinfo>BBa_K802004 short</partinfo>
 
<partinfo>BBa_K802004 short</partinfo>
  
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The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high plasmid copy number in both B. subtilis and E.coli.
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The shuttle vector of 6,5kb is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high plasmid copy number in both B. subtilis and E.coli. It has a polylinker containing all 4 of the iGEM sites.
  
  

Revision as of 16:00, 24 September 2012

Shuttle vector for E. coli and B. subtilis

The shuttle vector of 6,5kb is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high plasmid copy number in both B. subtilis and E.coli. It has a polylinker containing all 4 of the iGEM sites.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 6506
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6506
    Illegal NheI site found at 3377
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 6512
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6506
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 6506
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 6506
    Illegal XbaI site found at 6521
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3203
    Illegal BsaI.rc site found at 5190



Characterization

Transformation yield

In the NM522 E.coli strain the average yield was 3,3XE06 cells/µg.The transformed cells were selected on LB media supplemented with Ampicillin at a concentration of 100µg/mL.

In the BS168 B.subtilis strain the maximum yield was 70 cells/µg. The transformed cells were selected on LB media supplemented with Erythromycin at a concentration of 15µg/mL.

Antibiotic resistance

Sequencing of the modified regions