Difference between revisions of "Part:BBa K929102:Design"
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===Design Notes===
 
===Design Notes===
 
Part BBa_K929107 was used as template.
 
Part BBa_K929107 was used as template.
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===Source===
 
===Source===
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Nanobody: anti-GFP nanobody taken from Protein Data Bank(PDB: 3OGO), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)<br>
 
Nanobody: anti-GFP nanobody taken from Protein Data Bank(PDB: 3OGO), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)<br>
  
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Fc domain: human immunoglobulin gamma-1 heavy chain constant region taken from GenBank: J00228.1 (UniProt: P01857)
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Fc domain: human immunoglobulin gamma-1 heavy chain constant region taken from GenBank: J00228.1 (UniProt: P01857), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)<br>
  
 
===References===
 
===References===
 
Kubala MH, Kovtun O, Alexandrov K, Collins BM. (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci; 19, 2389-401
 
Kubala MH, Kovtun O, Alexandrov K, Collins BM. (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci; 19, 2389-401

Latest revision as of 11:22, 24 September 2012

Anti-GFP Nanobody with IgG kappa signal peptide and IgG1 Fc domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part BBa_K929107 was used as template.

Source

Signal peptide: human Ig kappa chain V-I region taken from UniProt P01601[1-22], gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

Nanobody: anti-GFP nanobody taken from Protein Data Bank(PDB: 3OGO), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

Fc domain: human immunoglobulin gamma-1 heavy chain constant region taken from GenBank: J00228.1 (UniProt: P01857), gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)

References

Kubala MH, Kovtun O, Alexandrov K, Collins BM. (2010) Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci; 19, 2389-401