Difference between revisions of "Part:BBa K929103:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K929103 short</partinfo> | <partinfo>BBa_K929103 short</partinfo> | ||
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TEV: TEV Protease recognition site according to life technologies manual (AcTEV TM Protease),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) | TEV: TEV Protease recognition site according to life technologies manual (AcTEV TM Protease),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) | ||
| − | LoxP sites: two for Cre mediated recombination, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) | + | LoxP sites: two for Cre mediated recombination, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) [1] |
Transmembrane domain: transmembrane domain of B-cell receptor with glycine-serine linker (BBa_K157010),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) | Transmembrane domain: transmembrane domain of B-cell receptor with glycine-serine linker (BBa_K157010),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) | ||
Reportergene: mcherry, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) | Reportergene: mcherry, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) | ||
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===References=== | ===References=== | ||
| + | [1] Araki K, Imaizumi T, Okuyama K, Oike Y, Yamamura KI (1997) Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters. J Biochem; 122, 977-82 | ||
Latest revision as of 10:36, 24 September 2012
B-cell transmembrane region with mCherry and TEV site, framed by LoxP
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus).
Source
TEV: TEV Protease recognition site according to life technologies manual (AcTEV TM Protease),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)
LoxP sites: two for Cre mediated recombination, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus) [1]
Transmembrane domain: transmembrane domain of B-cell receptor with glycine-serine linker (BBa_K157010),gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)
Reportergene: mcherry, gene synthesis by GeneArt, optimized for expression in CHO cells (Cricetulus griseus)
References
[1] Araki K, Imaizumi T, Okuyama K, Oike Y, Yamamura KI (1997) Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters. J Biochem; 122, 977-82