Difference between revisions of "Part:BBa K802004"

Revision as of 19:37, 23 September 2012

Shuttle vector for E. coli and B. subtilis

The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high plasmid copy number in both B. subtilis and E.coli.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal prefix found in sequence at 6506
Illegal suffix found in sequence at 1
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 6506
Illegal NheI site found at 3377
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 6512
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 6506
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found in sequence at 6506
Illegal suffix found in sequence at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found in sequence at 6506
Illegal XbaI site found at 6521
Illegal SpeI site found at 2
Illegal PstI site found at 16
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3203
Illegal BsaI.rc site found at 5190

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K802004 short</partinfo>
-The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high plasmid copy number in B. subtilis.
+The shuttle vector is Ampicillin resistant in E. coli and Erythromycin resistant in B. subtilis. It is a high plasmid copy number in both B. subtilis and E.coli.
 <!-- Add more about the biology of this part here
@@ Line 11: / Line 11: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K802004 SequenceAndFeatures</partinfo>
+== Construction ==
+== Characterization ==