Difference between revisions of "Part:BBa K785004:Design"
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<partinfo>BBa_K785004 short</partinfo> | <partinfo>BBa_K785004 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | The sequence is reverse translated from the protein | + | The sequence is reverse translated from the protein [http://www.rcsb.org/pdb/explore/explore.do?structureId=3P7N EL222] which is from Erythrobacter litoralis. The DNA is synthesized by Invitrogen company. |
===References=== | ===References=== | ||
Latest revision as of 18:20, 23 September 2012
Lov-HTH (light sensor with Ptet repression) light->PtetO repressed
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 633
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 187
Illegal AgeI site found at 297 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The HTH motif swap is the main issue in our design. We carefully choose the HTH sequence and swap it into the original amino acid sequence.
Source
The sequence is reverse translated from the protein [http://www.rcsb.org/pdb/explore/explore.do?structureId=3P7N EL222] which is from Erythrobacter litoralis. The DNA is synthesized by Invitrogen company.