Difference between revisions of "Part:BBa K808000"
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<partinfo>BBa_K808000 short</partinfo>
 
<partinfo>BBa_K808000 short</partinfo>
  
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This regulatory unit can be induced by L(+) - arabinose. The repressor Ara C is located upstream of the arabinose promoter.
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==Usage and Biology==
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L(+) - Arabinose is a sugar and ist harmless. For overexpression of proteins the concentration of 0,001-0,02% Arabinose can be used.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K808000 SequenceAndFeatures</partinfo>
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This sequenced biobrick  was alingned by using nucleotide NCBI BLAST with the original E. coli arabinose regulatory part of the arabinose degradation operon. The results provided 100% coverage.
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==Reference==
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Schleif, R. (2000). "Regulation of the L-arabinose operon of Escherichia coli." Trends Genet 16(12): 559-565.
  
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This regulatory unit can be induced by L(+) - arabinose. The repressor Ara C is located upstream of the arabinose promoter. L(+) - Arabinose is a sugar and is harmless.
 
−
For overexpression of proteins the concentration in range of 0,001-0,02% Arabinose can be used.
 
  
−
This sequenced biobrick  was alingned by using nucleotide NCBI BLAST (http://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&BLAST_SPEC=WGS&BLAST_PROGRAMS=megaBlast&PAGE_TYPE=BlastSearch) with the original E. coli arabinose regulatory part of the arabinose degradation operon. The results provided 100% coverage.
 
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 16:01, 23 September 2012

araC-Pbad - Arabinose inducible regulatory promoter/repressor unit

This regulatory unit can be induced by L(+) - arabinose. The repressor Ara C is located upstream of the arabinose promoter.


Usage and Biology

L(+) - Arabinose is a sugar and ist harmless. For overexpression of proteins the concentration of 0,001-0,02% Arabinose can be used.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


This sequenced biobrick was alingned by using nucleotide NCBI BLAST with the original E. coli arabinose regulatory part of the arabinose degradation operon. The results provided 100% coverage.


Reference

Schleif, R. (2000). "Regulation of the L-arabinose operon of Escherichia coli." Trends Genet 16(12): 559-565.