Difference between revisions of "Part:BBa K875001:Experience"

Revision as of 13:41, 23 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Trieste Team 2012

In liquid assay:

We inoculated a 20ml culture. After overnight growth, we diluted the culture to OD600 = 0.2. Then we aliquoted 200 μl in 8 replicates in a microtiter plate at different concentrations of cumate. The reading was performed in a monochromator at 485-510nm.

In the plate assay:

We streaked the culture on LB Agar plates containing different p-cumate concentrations.

User Reviews

UNIQ33bdfa337daecde5-partinfo-00000000-QINU UNIQ33bdfa337daecde5-partinfo-00000001-QINU

@@ Line 3: / Line 3: @@
 how you used this part and how it worked out.
-===Applications of BBa_K875001===
+'''Trieste Team 2012'''
-$wgFileExtensions[] = 'T5 Cumate operator _V.pdf';
+In liquid assay:
+We inoculated a 20ml culture. After overnight growth, we diluted the culture to OD600 = 0.2. Then we aliquoted 200 μl in 8 replicates in a microtiter plate at different concentrations of cumate. The reading was performed in a monochromator at 485-510nm.
+[[Image:T5opTS.png|frame|center|600px|alt="Graph 1"]]
+In the plate assay:
+We streaked the culture on LB Agar plates containing different p-cumate concentrations.
+[[Image:T5op2ts.png|frame|center|20px|alt="pic 1"]]
 ===User Reviews===