Difference between revisions of "Part:BBa K785003"
| Line 9: | Line 9: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | |||
| − | |||
| − | |||
[[Image:LUX expression 3.jpg|thumb|569px|center|The Lux light generator part expressed in the PET28a system(under the T7 lacO control). The Lux is induced by IPTG.]] | [[Image:LUX expression 3.jpg|thumb|569px|center|The Lux light generator part expressed in the PET28a system(under the T7 lacO control). The Lux is induced by IPTG.]] | ||
Revision as of 08:51, 23 September 2012
Lux(CDABEG) coding gene PoPS->Light
This page described the Lux operon from Vibrio fischeri. Lux gene is directly PCRed from the BBa_K325909, the pbad operon is deleted and now this part is available for any expression unit. You can provide light out put by putting this coding device under your promoter. We have successfully put this coding sequence into the PET28a expression system (cell strain is dH5α), and light out put could induced by the IPTG. To see more information about Lux light generator, please visit BBa_K325909, and a more complete description can be found on the [http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2010 Cambridge iGEM].