Difference between revisions of "Part:BBa K743014"
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[[Image:placa lux p.l.jpg|600px]]
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 23:48, 22 September 2012

after dusk -> LuxAB luciferase (Photorabdus luminiscens)

This part is designed for the expression of the LuxA and LuxB genes from Photorabdus luminiscens in a circadian manner, peaking a 2 hours after subjective dusk (or hour 14 of circadian time if you prefer). When transfromed into Syenchocystis PCC6803, the endogenous circadian transcriptional mechanism controls the activity of the Pta promoter making the heterodimeric LuxAB luciferase to be expressed at the specified time.

This permits for the emition of light by the luciferase reaction to be restricted to this time of the day (dependent on the addition or endogenous production of substrates)

Placa lux p.l.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 654
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1173