Difference between revisions of "Part:BBa K733005:Design"
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===Source=== | ===Source=== | ||
| − | Extract BMP-2 from mouse genomic DNA by PCR. Extract signal peptide YbdN from ''Bacillus subtilis'' genomic DNA by PCR. | + | Extract BMP-2 from mouse genomic DNA by PCR. Extract signal peptide YbdN from ''Bacillus subtilis'' genomic DNA by PCR. Ligation of YbdN and BMP2 achieved by overlapping PCR. |
===References=== | ===References=== | ||
Revision as of 18:11, 22 September 2012
ybdN+Bmp2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mouse BMP-2 DNA sequence has an EcoRI cutting site and this site is within the codon of the DNA sequence. To standardize the biobrick, we try to do a point mutation on mouse BMP-2 after we amplify it from PCR. The purpose is to remove the EcoRI cutting site and remain the correct codon for protein translation of BMP-2.
Source
Extract BMP-2 from mouse genomic DNA by PCR. Extract signal peptide YbdN from Bacillus subtilis genomic DNA by PCR. Ligation of YbdN and BMP2 achieved by overlapping PCR.