Difference between revisions of "Part:BBa K733009:Design"
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===Source=== | ===Source=== | ||
| − | + | We digest and ligate our pTms and BBa_E0240 from 2012 Kit Plate. | |
| + | |||
| + | Backbone: pTms in pSB1C3. Enzyme used: SpeI and PstI-HF. | ||
| + | |||
| + | Insert: BBa_E0240. Enzyme used: XbaI and PstI-HF. Purified by gel purification. | ||
===References=== | ===References=== | ||
Revision as of 17:19, 22 September 2012
Ptms+BBa_E0240: Ptms+RBS+GFP+Double
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 736
Design Notes
None.
Source
We digest and ligate our pTms and BBa_E0240 from 2012 Kit Plate.
Backbone: pTms in pSB1C3. Enzyme used: SpeI and PstI-HF.
Insert: BBa_E0240. Enzyme used: XbaI and PstI-HF. Purified by gel purification.