Difference between revisions of "Part:BBa K861047"
 
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<partinfo>BBa_K861047 short</partinfo>
 
<partinfo>BBa_K861047 short</partinfo>
  
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. FadI and FadJ are the anaerobic counterpart of FadA. They are shown to have better degradation of medium and short length fatty acid.
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FadI and FadJ are the anaerobic counterpart of FadA. They are shown to have better degradation efficiency of medium and short length fatty acids. We assembled FadI and FadJ with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.  
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Latest revision as of 14:30, 22 September 2012

PfadR regulated FadI and FadJ

FadI and FadJ are the anaerobic counterpart of FadA. They are shown to have better degradation efficiency of medium and short length fatty acids. We assembled FadI and FadJ with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]