Difference between revisions of "Part:BBa K907003"

Revision as of 10:12, 22 September 2012

Binary signal generator, promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS

<Part Description>

This part is composed of 3 elements.
1. RBSs: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter reversed
3. att sites: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase
We designed this part to generate two different proteins one at a time.

<Application>

At the first state, this device promotes the transcription and translation of upstream reversed gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence between with attB and attP sequence, promoter orientation changed. Then this device promotes the transcription and translation of downstream gene.

This device is tested by GFP and mRFP attached construct. See BBa_K0907004 and BBa_K0907005

Sequence and Features