Difference between revisions of "Part:BBa K907002"

Revision as of 10:12, 22 September 2012

Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS

<Part Description>

This part is composed of 3 elements.
1. RBSs: BBa_B0034, upsteam one is reversed
2. Promoter: BBa_J23119, Bacterial constitutive promoter
3. att sites: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase
We designed this part to generate two different proteins one at a time.

<Application>

At the first state, this device promotes the transcription and translation of downstream gene because of its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts the sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes the transcription and translation of upstream gene(must be reversed at the construction step).

The promoter reversed version is BBa_K907003.
This device is tested by GFP and mRFP attached construct. See BBa_K0907004 and BBa_K0907005

Sequence and Features