Difference between revisions of "Part:BBa K861061"

Latest revision as of 09:18, 22 September 2012

Efficacy testing RFP generator of BBa_K861060 (PfadR)

We placed BBa_I13507 downstream of BBa_K861060 (PfadR) in plasmid pSB6A1. M9 medium with oleic acid as sole carbon source was used to test the efficacy of the promoter. Specifically, oleic acid was emulsified with 10% Triton X100 with volume 1:1. Then various volume of mixture is added to M9 medium with 0.2% triton X100. We vortex E.coli Dh5α in the medium for 24h and using SpectraMax M2 plate reader to see its OD 600 and fluorescence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 657
Illegal AgeI site found at 769
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K861061 short</partinfo>
-We use BBa_I13507 test function of BBa_K861060 (PfadR) in M9 medium with oleic acid as sole carbon source in plasmid pSB6A1. Specifically, oleic acid was emulsified with 10% Triton X100 with volume 1:1. Then various volume of mixture is added to M9 medium with 0.2% triton X100. We vortex E.coli Dh5 alpha in the medium for 24h and using SpectraMax M2 plate reader to see its OD600 and fluorescence.
+We placed BBa_I13507 downstream of BBa_K861060 (PfadR) in plasmid pSB6A1. M9 medium with oleic acid as sole carbon source was used to test the efficacy of the promoter. Specifically, oleic acid was emulsified with 10% Triton X100 with volume 1:1. Then various volume of mixture is added to M9 medium with 0.2% triton X100. We vortex E.coli Dh5α in the medium for 24h and using SpectraMax M2 plate reader to see its OD 600 and fluorescence.
 <!-- Add more about the biology of this part here