Difference between revisions of "Part:BBa K861048"

 
 
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<partinfo>BBa_K861048 short</partinfo>
 
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S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that they have far better efficiency compared to those in E.coli. We assembled S-FadA and S-FadB with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.
  
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S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that S-FadA have far better efficiency compared to FadA in E.coli.This device contains S-FadA and S-FadB with a RBS and a terminator.
 
  
 
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Latest revision as of 08:42, 22 September 2012

PfadR regulated S-FadA and S-FadB

S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that they have far better efficiency compared to those in E.coli. We assembled S-FadA and S-FadB with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1539