Difference between revisions of "Part:BBa K861046"
 
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<partinfo>BBa_K861046 short</partinfo>
 
<partinfo>BBa_K861046 short</partinfo>
  
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. In the process of LCFA degradation,the cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins.
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In the process of LCFA degradation,the cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins. We assembled FadA and FadB with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:39, 22 September 2012

PfadR regulated FadA and FadB

In the process of LCFA degradation,the cycles of hydration, oxidation, and thiolytic cleavage are carried out by tetrameric complex consisting of two FadA and two FadB proteins. We assembled FadA and FadB with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 188
    Illegal BglII site found at 1064
    Illegal BglII site found at 1447
    Illegal BglII site found at 1594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 678
  • 1000
    COMPATIBLE WITH RFC[1000]