Difference between revisions of "Part:BBa K821001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | We mutated the Shine-Dalgarno sequence from the native RBS B0034 to the orthogonal RBS 1 BBa_K821009. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | From BioBrick | + | From BioBrick BBa_J04450 |
===References=== | ===References=== | ||
Lon M. Chubiz,Christopher V. Rao, Computational design of orthogonal ribosomes, Nucleic acids research, Vol. 36, No. 12, 4038–4046 | Lon M. Chubiz,Christopher V. Rao, Computational design of orthogonal ribosomes, Nucleic acids research, Vol. 36, No. 12, 4038–4046 | ||
Latest revision as of 06:03, 22 September 2012
RFP Reporter with o-RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 769
Illegal AgeI site found at 881 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We mutated the Shine-Dalgarno sequence from the native RBS B0034 to the orthogonal RBS 1 BBa_K821009.
Source
From BioBrick BBa_J04450
References
Lon M. Chubiz,Christopher V. Rao, Computational design of orthogonal ribosomes, Nucleic acids research, Vol. 36, No. 12, 4038–4046