Difference between revisions of "Part:BBa K861048"

Revision as of 16:46, 21 September 2012

PfadR regulated S-FadA and S-FadB

S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that they have far better efficiency compared to those in E.coli. We assebled S-FadA and S-FadB with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 189
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2309
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1539

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 <partinfo>BBa_K861048 short</partinfo>
-To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that they have far better efficiency compared to those in E.coli.
+S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that they have far better efficiency compared to those in E.coli. We assebled S-FadA and S-FadB with PfadR (BBa_K861060) hoping they will only be overexpressed when fatty acids concentration is high.
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