Difference between revisions of "Part:BBa K861048"

 
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<partinfo>BBa_K861048 short</partinfo>
 
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To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that they have far better efficiency compared to those in E.coli.
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S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that S-FadA have far better efficiency compared to FadA in E.coli.This device contains S-FadA and S-FadB with a RBS and a terminator.
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Revision as of 16:27, 21 September 2012

PfadR regulated S-FadA and S-FadB

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins, put them in different combination with substrates needed for fatty acid oxidation in a test tube to look for the combination that have best degradation capability. S-FadA gene and S-FadB gene from S. enterica function as FadA and FadB in E.coli. Yet it is shown that they have far better efficiency compared to those in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 189
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1539